Call Us
Home » Products » Elisa Test Kits » Hepatitis B Virus ELISA Kit

Hepatitis B Virus ELISA Kit

Hepatitis B Virus ELISA Kit
Hepatitis B Virus ELISA Kit
Send Inquiry
Product Code : 02
Brand Name : Bioneovan
  • Equipment Type
  • Test Kit
  • Payment Terms
  • Paypal, Telegraphic Transfer (T/T), Letter of Credit at Sight (Sight L/C), Cash in Advance (CID), Cash Advance (CA), Cash on Delivery (COD), Cash Against Delivery (CAD)
  • Supply Ability
  • 10000 Box/Boxes per Month 25 workdays10000 Box/Boxes per Month 25 workdays Per Month
  • Delivery Time
  • 15 Days
  • Sample Policy
  • Sample costs shipping and taxes has to be paid by the buyer
  • Main Export Market(s)
  • Australia, Asia
  • Main Domestic Market
  • All India
Product Description

Our Hepatitis B Virus ELISA Kit is a sandwich enzyme immunoassay for the qualitative detection of HBsAg in human serum or plasma. The ELISA Kit is designed as an aid in screening blood donors and an aid in the diagnosis of clinical conditions related to hepatitis B-infected individuals. The micro titer well-plate in this kit has been pre-coated and blocked. Samples or controls are added to the plate wells, incubated and washed.

Features:

  • Exceptional value for money
  • Rapid detection
  • Highly specific detection profile
  • High priming efficiency
  • Broad dynamic detection range
  • Accurate controls to confirm findings

Test Procedure;

  1. Bring ELISA Kit (all reagents), and Specimens to room temperature before use (approximately 30 minutes).
  2. Dilute concentrated wash buffer 1:19 with ddH2O.
  3. For each test, set one blank, two positive and three negative controls. Add 100μl positive and negative control serum into positive and negative control wells respectively.
  4. Add 100 μl sample diluents in each other test wells, then at 10 μl test serum into test wells. Pipet up and down to mix the samples well.
  5. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  6. Discard the liquid in all wells and fill the wells with wash solution. Lay aside for 15 seconds, discard the liquid in all wells and fill the wells with wash solution. Repeat 5 times and dry wells after last wash.
  7. Add 100 μl Enzyme Conjugant in each well except the blank.
  8. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  9. Repeat step 6.
  10. Add 50μl substrate A and B respectively to each well including the blank well. Mix gently, protected from light and incubates 15 minutes at 37°C.
  11. Add 50μl of stop solution into each well to stop the reaction, including blank well.
  12. Measure the absorbance at 450 nm against the blank, or measure the absorbance at 450 nm/630-690 nm.

BIONEOVAN CO., LTD.
X


Back to top
BIONEOVAN CO., LTD. All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Ltd.