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HEV Elisa Test Kit

HEV Elisa Test Kit
HEV Elisa Test Kit
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Product Code : 01
Brand Name : Bioneovan
Price And Quantity
  • Minimum Order Quantity
  • 10000 Box/Boxes per Month 25 workdays
  • Unit of Measure
  • Case/Cases
Product Specifications
  • Equipment Type
  • Test Kit
Product Description

Our HEV Elisa Test Kit assays are dynamic research tools and have high sensitivity and excellent specificity for detection of human hepatitis E virus antibody. No significant cross-reactivity or interference between human hepatitis E virus antibody and analogues was observed. It employs the qualitative enzyme immunoassay technique. The micro titer plate provided in this kit has been pre-coated with an antigen. Any antibodies specific for the antigen will bind to the pre-coated antigen.

Features

  • User-friendly directions and explanation of test procedures
  • Simple and safe reagent preparation
  • Clear instructions on specimen collection
  • Comprehensive package of required materials
  • Explicit quality control and storage guidelines
  • Reliable and easy-to-read test results

TEST PROCEDURE

  1. Bring ELISA Kit (all reagents), and Specimens to room temperature before use (approximately 30 minutes).
  2. Dilute concentrated wash buffer 1:19 with ddH2O.
  3. For each test, set one blank, two positive and three negative controls. Add 100μl positive and negative control serum into positive and negative control wells respectively.
  4. Add 100 μl sample diluents in each other test wells, then at 10 μl test serum into test wells. Pipet up and down to mix the samples well.
  5. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  6. Discard the liquid in all wells and fill the wells with wash solution. Lay aside for 15 seconds, discard the liquid in all wells and fill the wells with wash solution. Repeat 5 times and dry wells after last wash.
  7. Add 100 μl Enzyme Conjugant in each well except the blank.
  8. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  9. Repeat step 6.
  10. Add 50μl substrate A and B respectively to each well including the blank well. Mix gently, protected from light and incubates 15 minutes at 37°C.
  11. Add 50μl of stop solution into each well to stop the reaction, including blank well.
  12. Measure the absorbance at 450 nm against the blank, or measure the absorbance at 450 nm/630-690 nm.


Trade Information
  • Payment Terms
  • Paypal, Letter of Credit at Sight (Sight L/C), Cash on Delivery (COD), Cash Against Delivery (CAD)
  • Supply Ability
  • 10000 Box/Boxes per Month 25 workdays Per Month
  • Delivery Time
  • 15 Days
  • Sample Policy
  • Sample costs shipping and taxes has to be paid by the buyer
  • Main Export Market(s)
  • Middle East, Australia, Asia
  • Main Domestic Market
  • All India

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