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HIV Aids Elisa Test Kit

HIV Aids Elisa Test Kit
HIV Aids Elisa Test Kit
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Product Code : 09
Brand Name : Bioneovan
Price And Quantity
Product Specifications
  • Equipment Type
  • Test Kit
Product Description

The enzyme-linked immunosorbent assay (ELISA) or EIA, detects HIV antibodies and antigens in the blood. With our offered HIV Aids Elisa Test Kit blood tests can be used to diagnose HIV by detecting certain antibodies or proteins produced by the immune system in response to the virus. The assay can be utilized for screening of blood donors or as an aid in the diagnosis of clinical conditions related to infection with HIV.

ELISA test kit may be used to diagnose:

  • HIV, which causes AIDS
  • Lyme disease
  • Pernicious anemia
  • Rocky Mountain spotted fever
  • Rotavirus
  • Squamous cell carcinoma
  • Syphilis
  • Toxoplasmosis
  • Varicella-zoster virus, which causes chickenpox and shingles
  • Zika virus

Test Procedure:

  1. Bring ELISA Kit (all reagents), and Specimens to room temperature before use (approximately 30 minutes).
  2. Dilute concentrated wash buffer 1:19 with ddH2O.
  3. For each test, set one blank, two positive and three negative controls. Add 100μl positive and negative control serum into positive and negative control wells respectively.
  4. Add 100 μl sample diluents in each other test wells, then at 10 μl test serum into test wells. Pipet up and down to mix the samples well.
  5. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  6. Discard the liquid in all wells and fill the wells with wash solution. Lay aside for 15 seconds, discard the liquid in all wells and fill the wells with wash solution. Repeat 5 times and dry wells after last wash.
  7. Add 100 μl Enzyme Conjugant in each well except the blank.
  8. Cover wells with seal paper, and then incubate 30 minutes at 37°C.
  9. Repeat step 6.
  10. Add 50μl substrate A and B respectively to each well including the blank well. Mix gently, protected from light and incubates 15 minutes at 37°C.
  11. Add 50μl of stop solution into each well to stop the reaction, including blank well.
  12. Measure the absorbance at 450 nm against the blank, or measure the absorbance at 450 nm/630-690 nm.

 

Trade Information
  • Payment Terms
  • Paypal, Telegraphic Transfer (T/T), Letter of Credit at Sight (Sight L/C), Cash in Advance (CID), Cash Against Delivery (CAD)
  • Supply Ability
  • 10000 Box/Boxes per Month 25 workdays Per Month
  • Delivery Time
  • 15 Days
  • Sample Policy
  • Sample costs shipping and taxes has to be paid by the buyer
  • Main Export Market(s)
  • North America, Central America, Asia
  • Main Domestic Market
  • All India

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