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Troponin I(cTnI) Test cassette

Troponin I(cTnI) Test cassette
Troponin I(cTnI) Test cassette
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Product Code : 51
Product Description

INTRODUCTION

Troponin I (cTnl) Test is a qualitative, membrane-based immunoassay for the detection of the cTnl present in the whole blood, serum or plasma. The membrane is pre-coated with streptavidin on the test line region of the kit.

PRINCIPLE

During testing, the whole blood, serum or plasma specimen reacts with the particle coated with anti-cTnl antibodies and biotinylated anti-cTnl antibodies. The mixture migrates upward on the membrane through chromatography by capillary action to react with the streptavidin present on the membrane and develop a red line. The presence of this red line in the test region indicates a positive result, while its absence indicates a negative result. A red line will always appear in the control line region (C) it serves as a procedural control and indicates that proper volume of specimen has been added and the membrane wicking has occurred.

TEST PROCEDURE

  • Remove the test card from the pouch and allow it to equilibrate to room temperature prior to testing. Place the card flat on a clean, dry and non-absorbent surface.

  • Dispense 2 drops of serum specimen (100ul) into the specimen well.

  • Wait for 20 minutes and read the result. Do not read the results after 20 minutes.

INTERPRETATION OF RESULTS

  • Negative: Only 1 colored band appears on the control (C) region and no apparent band on the test (T) region.

  • Positive: In addition to a pink control (C) band, a distinct pink band also appears in the test (T) region.

  • Invalid: Absence of color in both regions indicates a procedural error and/or the test reagent has deteriorated.

STORAGE AND STABILITY

The test kit should be stored at room temperature between 4 to 30° C in the sealed pouch until the date of expiry. The test kit should be kept away from direct sunlight, moisture, and heat.



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