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Troponin I (cTnl) Test is a qualitative, membrane-based immunoassay for the detection of the cTnl present in the whole blood, serum or plasma. The membrane is pre-coated with streptavidin on the test line region of the kit.
During testing, the whole blood, serum or plasma specimen reacts with the particle coated with anti-cTnl antibodies and biotinylated anti-cTnl antibodies. The mixture migrates upward on the membrane through chromatography by capillary action to react with the streptavidin present on the membrane and develop a red line. The presence of this red line in the test region indicates a positive result, while its absence indicates a negative result. A red line will always appear in the control line region (C) it serves as a procedural control and indicates that proper volume of specimen has been added and the membrane wicking has occurred.
Remove the test card from the pouch and allow it to equilibrate to room temperature prior to testing. Place the card flat on a clean, dry and non-absorbent surface.
Dispense 2 drops of serum specimen (100ul) into the specimen well.
Wait for 20 minutes and read the result. Do not read the results after 20 minutes.
INTERPRETATION OF RESULTS
Negative: Only 1 colored band appears on the control (C) region and no apparent band on the test (T) region.
Positive: In addition to a pink control (C) band, a distinct pink band also appears in the test (T) region.
Invalid: Absence of color in both regions indicates a procedural error and/or the test reagent has deteriorated.
STORAGE AND STABILITY
The test kit should be stored at room temperature between 4 to 30° C in the sealed pouch until the date of expiry. The test kit should be kept away from direct sunlight, moisture, and heat.